The isolates differed in their ability to ferment L-arabinose, D-dulcitol, D-sorbitol, D-xylose and in the production of indole and H2S in triple sugar iron agar resulting in the identification of 8 biochemical types or biovars. All isolates also fermented D-glucose, D-mannitol, and sucrose but failed to ferment lactose. Consistent results were obtained for all isolates in the test for Gram reaction, oxidase, catalase, urease, no growth on MacConkey agar and nitrate reduction. The field isolates were obtained in Nigeria from chickens (15 isolates), quail (5 isolates), cattle (31 isolates), goats (7 isolates), sheep (8 isolates), rabbits (3 isolates) and the vaccine strains (3 isolates), which are used as prophylaxis against fowl cholera and haemorrhagic septicaemia diseases. Phenotypic diversity among 69 field isolates plus 3 vaccine strains previously identified as Pasteurella multocida were investigated by extended phenotypic characterization. multocida type A ToxA(+) in respiratory infection among sheep. multocida type A ToxA(+) in diseased sheep found in this study is noticeable and attribute to important role of P. The high prevalence of ToxA and TbPA among diseased sheep may imply to important role of these genes in epidemiological and virulence of P. Among 4 virulence genes detected by PCR we found a remarkable high prevalence of TbPA (69.4%) and ToxA (72.2%) genes in diseased animals. Capsular typing of isolates by PCR demonstrated two capsular types A (39), D (3) including 5 untyped with 83.3%, 6.3% and 10.6% prevalence, respectively. Forty seven isolates of Pasteurella multocida were isolated from clinically healthy and diseased sheep. To learn more about the virulence of Pasteurella multocida isolated from sheep, phenotype, capsular type and some virulence factors (Pfha1, HgbB, TbPA and ToxA) of ovine Pasteurella multocida is described in this study. The study has been the sensitive levels of Ribo-PCR in epidemiological studies of pasteurellosis, however, correlation among diversity of isolates and host origin is not fully understood. multocida and understanding their interactions with host. Molecular approaches such as Ribo-PCR are necessary for determining and characterizing the diversity of P. Cluster II was divided in four sub cluster including IIa, IIb, IIc and IId. Two minor cluster I and II was obtained with no significant diversity among the isolates. Nine isolates including JF694004.1 and 8 isolates including JF681973.1 showed 100% and 94% similarity, respectively. Capsular type A was dominant among the isolates and was variable than serogroup D. Twenty samples representing Pasteurella phenotypes by Entero rapid kit and 17 out of 20 isolates were identified as P.multocida. They were analyzed by biochemical tests and Ribo-PCR 16S-23S ribosomal RNA genes. A total of 120 swab tonsil and nasal samples were obtained from sheep and goats. Ribotyping PCR (Ribo-PCR) was used for investigating the diversity of ovine and caprine P.multocida. Such results, the authors believe, demonstrate that adequate risk assessments should be undertaken in Turkey and neighbouring countries.Pasteurella multocida is considered to be an important cause of ovine pneumonia and results causes considerable economic losses in Iran. It may also indicate that Mccp presents a risk for wildlife in the region. The isolation of Mccp from sheep lung lesions brings the strict host-specificity of this pathogen into question. The East Turkish isolate was found to be closely related to another strain of Turkish origin, as well as to Mccp strains isolated in Tunisia. capripneumoniae was isolated in pure culture and characterised at a finer molecular level. This agent was also isolated from two of 13 sheep samples (one from the lung and the other from a nasal swab). The results showed that the agent of CCPP, Mycoplasma capricolum subspecies capripneumoniae (Mccp), could be detected by culture and specific polymerase chain reaction from 37.5% (12/32) of lung samples taken from goats of ten different herds. This study was based on clinical surveillance in the field, surveillance at regional slaughterhouses and regular submission of suspected lesions to regional laboratories. A study was implemented to investigate the presence of contagious caprine pleuropneumonia (CCPP) in East Turkey.
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